293

 
Species source: human
Organ source: kidney; fetal
Plasmid:
  • pcDNA3
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 500μg/ml - 7-10days
Reference:
  • J. R. Jasper, J. D. Lesnick, L. K. Chang, S. S. Yamanishi, T. K. Chang, S. A. O. Hsu, D. A. Daunt, D. W. Bonhaus and R. M. Eglen 1998. Ligand Efficacy and Potency at Recombinant [alpha] 2 Adrenergic Receptors:: Agonist-Mediated [35s] gtp [gamma] s Binding