PC12

 
Plasmid:
  • pCXNII
  • pCXNII/GnT-III
  • pCXNII/D321AGnT-III
Culture/Media: Dulbecco's modified Eagle's medium
Atmosphere: 5%CO2
Antibiotics:
  • Geneticin (G418) - 1000μg/ml -
Reference:
  • J. Gu, Y. Zhao, T. Isaji, Y. Shibukawa, H. Ihara, M. Takahashi, Y. Ikeda, E. Miyoshi, K. Honke and N. Taniguchi 2004. 1, 4-N-Acetylglucosaminyltransferase III down-regulates neurite outgrowth induced by costimulation of epidermal growth factor and integrins through the Ras/ERK signaling pathway in PC12 cells