MDCK (NBL-2)

 
Species source: dog
Organ source: kidney
Plasmid:
  • pEGFP-C3
  • pcDNA3.1
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 800μg/ml - 2weeks
Reference:
  • D. Michaelson, J. Silletti, G. Murphy, P. D'Eustachio, M. Rush and M. R. Philips 2001. Differential Localization of Rho Gtpases in Live Cells