HeLa

 
Species source: human
Organ source: cervix; adenocarcinoma
Plasmid:
  • pSUPER-shRNA
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Puromycin - 1.5μg/ml -
Reference:
  • X. Liu, M. Vorontchikhina, Y. L. Wang, F. Faiola and E. Martinez 2008. STAGA recruits Mediator to the MYC oncoprotein to stimulate transcription and cell proliferation